| Descrição:
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Programa:
• Edição genética em Zebrafish - histórico e potencial.
• Microinjeção de embriões de Zebrafish.
• Novo mecanismo de robustez genética: Adaptação transcricional.
Referências:
Carrington B, Bishop K, Sood R. A Comprehensive Review of Indel Detection Methods for Identification of Zebrafish Knockout Mutants Generated by Genome-Editing Nucleases. Genes (Basel). 2022 May 11;13(5):857. doi: 10.3390/genes13050857.
Shin, Unbeom, et al. "Large-scale generation and phenotypic characterization of zebrafish CRISPR mutants of DNA repair genes." DNA repair 107 (2021): 103173.
Tromp A, Robinson K, Hall TE, Mowry B, Giacomotto J. Pipeline for generating stable large genomic deletions in zebrafish, from small domains to whole gene excisions. G3 (Bethesda). 2021 Dec 8;11(12):jkab321. doi: 10.1093/g3journal/jkab321.
Uribe-Salazar JM, Kaya G, Sekar A, Weyenberg K, Ingamells C, Dennis MY. Evaluation of CRISPR gene-editing tools in zebrafish. BMC Genomics. 2022 Jan 6;23(1):12. doi: 10.1186/s12864-021-08238-1.
Vejnar CE, Moreno-Mateos MA, Cifuentes D, Bazzini AA, Giraldez AJ. Optimized CRISPR-Cas9 System for Genome Editing in Zebrafish. Cold Spring Harb Protoc. 2016 Oct 3;2016(10). doi: 10.1101/pdb.prot086850. |