|
|
Atividade
| 130362 - Diferenciação celular e biomineralização |
| Período da turma: | 01/03/2025 a 31/03/2025
|
||||
|
|
|||||
| Descrição: | Esta discipliina tem como objetivo apresentar os mecanismos envolvidos na diferenciação de células-tronco envolvidas na formação do dente e dos tecidos de suporte, assim como de células envolvidas no catabolismo de tecidos mineralizados.
Conteúdo programático: 1) Cultura de células eucarióticas - Tipos de culturas: células primárias e linhagens celulares. - Modo de crescimento: células aderentes ou em suspensão. - Meios de cultivo e suplementações utilizadas. - Avaliação microscópica das células em cultura. - Congelamento, descongelamento e armazenamento celular. - Contaminação da cultura: uso de antibióticos e antifúngicos. 2) Métodos laboratoriais para detecção de ácidos nucleicos, proteínas e enzimas - Análise do DNA e da expressão gênica (RNA): reação em cadeia da polimerase (PCR) em tempo real e silenciamento de RNAm. - Análise da síntese de proteínas intra e extra-celulares: ensaio imunoenzimático (ELISA), western blotting, imunocitoquímica, imunohistoquímica, imunofluorescência. - Análise da atividade enzimática: fosfatase ácida resistente ao tartarato (TRAP), zimografia convencional, zimografia in situ. 3) Pesquisa translacional - Modelos animais utilizados em pesquisas pré-clínicas em Odontologia. - Papel dos mediadores inflamatórios na inflamação e no reparo ósseo e dentinário. -Reguladores da osteoclastogênese, reabsorção óssea e reabsorção dentária como potenciais estratégias terapêuticas inovadoras. - Modelos in vivo e ensaios funcionais para investigação do processo de biomineralização. Referências - Almeida-Junior LA, de Campos Chaves Lamarque G, Herrera H, Arnez MFM, Lorencetti-Silva F, Silva RAB, Silva LAB, Paula-Silva FWG. Analysis of the cytotoxicity and bioactivity of CeraSeal, BioRoot™ and AH Plus® sealers in pre-osteoblast lineage cells. BMC Oral Health. 2024 Feb 22;24(1):262. doi: 10.1186/s12903-024-04021-2. PubMed PMID: 38389110. - Almeida-Junior LA, Araujo LDC, Lamarque GCC, Arnez MFM, Kapila YL, Silva LABD, Paula-Silva FWG. Reparative Dentin Formation Following Dental Pulp Capping is Mediated by TNFR1 In Vivo. J Endod. 2023 Oct;49(10):1329-1336. doi: 10.1016/j.joen.2023.06.015. PubMed PMID: 37423584. - Almeida-Junior LA, de Carvalho MS, Almeida LKY, Silva-Sousa AC, Sousa-Neto MD, Silva RAB, Silva LAB, Paula-Silva FWG. TNF-α-TNFR1 Signaling Mediates Inflammation and Bone Resorption in Apical Periodontitis. J Endod. 2023 Oct;49(10):1319-1328.e2. doi: 10.1016/j.joen.2023.07.013. PubMed PMID: 37499863. - Barbosa PIZ, Lima RB, Marubayashi LM, Oliveira HF, Silva RABD, Nelson-Filho P, Arnez MFM, Paula-Silva FWG, Queiroz AM. Activation of gelatinases in permanent human teeth after different experimental radiotherapy protocols. Braz Dent J. 2023 Nov-Dec;34(6):130-139. doi: 10.1590/0103-6440202305542. PubMed PMID: 38133087. - de Carvalho MS, de Almeida-Junior LA, Ribeiro YJS, Arnez MFM, da Silva RAB, de Carvalho FK, Paula-Silva FWG. Detection of Apical Dental Resorption Caused by Endodontic Infection in Mice Using Fluorescence and Bright-Field Microscopy. Scanning. 2023;2023:4619503. doi: 10.1155/2023/4619503. eCollection 2023. PubMed PMID: 37101708; PubMed Central PMCID: PMC10125736. - Petean IBF, Silva-Sousa AC, da Silva RAB, Lucisano MP, da Silva LAB, de Castro GPA, Sousa-Neto MD, Faccioli LH, Paula-Silva FWG. Systemic inhibition of 5-lipoxygenase by MK-886 exacerbates apical periodontitis bone loss in a mouse model. BMC Oral Health. 2023 Jan 10;23(1):11. doi: 10.1186/s12903-023-02712-w. PubMed PMID: 36624436; PubMed Central PMCID: PMC9830793. - de Castro GAP, Petean IBF, de Paula-Silva FWG, Kuchler EC, Antunes LDS, Segato RAB, da Silva LAB, Silva-Sousa AC, Sousa-Neto MD. Genetic polymorphism in the tumour necrosis factor alpha gene (G-308A) is associated with persistent apical periodontitis in Brazilians. Int Endod J. 2023 Jan;56(1):17-26. doi: 10.1111/iej.13841. Epub 2022 Oct 17. PubMed PMID: 36183324. - de Carvalho MS, de Almeida-Junior LA, Silva-Sousa AC, Sousa-Neto MD, Lucisano MP, Arnez MFM, da Silva LAB, Paula-Silva FWG. Absence of Tumor Necrosis Factor Receptor 1 Inhibits Osteoclast Activity in Apical Dental Resorption Caused by Endodontic Infection in Mice. J Endod. 2022 Nov;48(11):1400-1406. doi: 10.1016/j.joen.2022.08.003. Epub 2022 Aug 12. PubMed PMID: 35964707. - Gonçalves JL, Duarte ACA, Almeida-Junior LA, de Carvalho FK, de Queiroz AM, Arnez MFM, Faccioli LH, Paula-Silva FWG. Enamel biomineralization under the effects of indomethacin and celecoxib non-steroidal anti-inflammatory drugs. Sci Rep. 2022 Sep 22;12(1):15823. doi: 10.1038/s41598-022-19583-w. PubMed PMID: 36138112; PubMed Central PMCID: PMC9500046. - Lorencetti-Silva F, Arnez MFM, Thomé JPQ, Carvalho MS, Carvalho FK, Queiroz AM, Faccioli LH, Paula-Silva FWG. Leukotriene B4 Loaded in Microspheres Inhibits Osteoclast Differentiation and Activation. Braz Dent J. 2022 Sep-Oct;33(5):35-45. doi: 10.1590/0103-6440202204827. PubMed PMID: 36287497; PubMed Central PMCID: PMC9645171. - Arnez MFM, Monteiro PM, Paula-Silva FWG, Dessotti GB, Menezes LM, Küchler EC, Alves SYF, Matsumoto MAN, Stuani MBS. Impact of cigarette smoke on osteogenic and osteoclast signaling in middle palatal suture. Braz Dent J. 2022 Mar-Apr;33(2):99-108. doi: 10.1590/0103-6440202203966. PubMed PMID: 35508042. - Petean IBF, Silva-Sousa AC, Cronenbold TJ, Mazzi-Chaves JF, Silva LABD, Segato RAB, Castro GAP, Kuchler EC, Paula-Silva FWG, Sousa-Neto MD. Genetic, Cellular and Molecular Aspects involved in Apical Periodontitis. Braz Dent J. 2022 Jul-Aug;33(4):1-11. doi: 10.1590/0103-6440202205113. Review. PubMed PMID: 36043561; PubMed Central PMCID: PMC9645190. - da Silva FL, de Campos Chaves Lamarque G, de Oliveira FMMPC, Nelson-Filho P, da Silva LAB, Segato RAB, Faccioli LH, Paula-Silva FWG. Leukotriene B4 loaded in microspheres regulate the expression of genes related to odontoblastic differentiation and biomineralization by dental pulp stem cells. BMC Oral Health. 2022 Feb 23;22(1):45. doi: 10.1186/s12903-022-02083-8. PubMed PMID: 35197043; PubMed Central PMCID: PMC8864908. - Taira TM, Ribeiro VL, Ribeiro YJS, da Silva RAB, da Silva LAB, Politi MPL, Faccioli LH, Paula-Silva FWG. Gene expression data of inflammatory mediators in apical periodontitis in 129 (wild type) and 5-lipoxygenase knockout mice. Data Brief. 2022 Feb;40:107787. doi: 10.1016/j.dib.2021.107787. eCollection 2022 Feb. PubMed PMID: 35071699; PubMed Central PMCID: PMC8762048. - Olsson B, da Silva MJ, Lago C, Calixto RD, Ramazzotto LA, Barbosa Rebellato NL, Kirschneck C, Garcia Paula-Silva FW, Küchler EC, Scariot R. Single Nucleotide Polymorphisms in Runt-related Transcription Factor 2 and Bone Morphogenetic Protein 2 Impact on Their Maxillary and Mandibular Gene Expression in Different Craniofacial Patterns - A Comparative Study. Ann Maxillofac Surg. 2021 Jul-Dec;11(2):222-228. doi: 10.4103/ams.ams_40_21. Epub 2022 Feb 1. PubMed PMID: 35265489; PubMed Central PMCID: PMC8848693. - Ribeiro-Santos FR, Arnez MFM, de Carvalho MS, da Silva RAB, Politi MPL, de Queiroz AM, Nelson-Filho P, da Silva LAB, Faccioli LH, Paula-Silva FWG. Effect of non-steroidal anti-inflammatory drugs on pulpal and periapical inflammation induced by lipopolysaccharide. Clin Oral Investig. 2021 Nov;25(11):6201-6209. doi: 10.1007/s00784-021-03919-3. Epub 2021 Apr 1. PubMed PMID: 33791868. - Sorgi CA, de Campos Chaves Lamarque G, Verri MP, Nelson-Filho P, Faccioli LH, Paula-Silva FWG. Multifaceted effect of caffeic acid against Streptococcus mutans infection: microbicidal and immunomodulatory agent in macrophages. Arch Microbiol. 2021 Aug;203(6):2979-2987. doi: 10.1007/s00203-021-02290-x. Epub 2021 Mar 26. PubMed PMID: 33772326. - Petean IBF, Almeida-Junior LA, Arnez MFM, Queiroz AM, Silva RAB, Silva LAB, Faccioli LH, Paula-Silva FWG. Celecoxib treatment dampens LPS-induced periapical bone resorption in a mouse model. Int Endod J. 2021 Aug;54(8):1289-1299. doi: 10.1111/iej.13472. Epub 2021 May 28. PubMed PMID: 33403674. - Paula-Silva FWG, Arnez MFM, de Campos Chaves Lamarque G, Petille R, Ribeiro-Santos FR, de Sena MF, Nelson-Filho P, da Silva LAB. Osteoclast formation, inflammation, and matrix metalloproteinase-9 are downregulated in bone repair following root canal treatment in dogs teeth. Clin Oral Investig. 2021 Jul;25(7):4699-4707. doi: 10.1007/s00784-021-03784-0. Epub 2021 Jan 15. PubMed PMID: 33449195. - da Silva LAB, Longo DL, Stuani MBS, de Queiroz AM, da Silva RAB, Nelson-Filho P, Vieira HAO, Pucinelli CM, Paula-Silva FWG. Effect of root surface treatment with denusomab after delayed tooth replantation. Clin Oral Investig. 2021 Mar;25(3):1255-1264. doi: 10.1007/s00784-020-03430-1. Epub 2020 Jul 29. PubMed PMID: 32728940. - Silva LABD, Hidalgo LRDC, de Sousa-Neto MD, Arnez MFM, Barnett F, Hernández PMG, Faccioli LH, Paula-Silva FWG. Cytotoxicity and Inflammatory Mediators Release by Macrophages Exposed to Real Seal XT and Sealapex Xpress. Braz Dent J. 2021 Jan-Feb;32(1):48-52. doi: 10.1590/0103-6440202103330. PubMed PMID: 33914002. - Paula-Silva FWG, Ribeiro-Santos FR, Petean IBF, Manfrin Arnez MF, Almeida-Junior LA, Carvalho FK, Silva LABD, Faccioli LH. Root canal contamination or exposure to lipopolysaccharide differentially modulate prostaglandin E 2 and leukotriene B 4 signaling in apical periodontitis. J Appl Oral Sci. 2020;28:e20190699. doi: 10.1590/1678-7757-2019-0699. Epub 2020 May 11. PubMed PMID: 32401938; PubMed Central PMCID: PMC7213784. - Paula-Silva FWG, Arnez MFM, Petean IBF, Almeida-Junior LA, da Silva RAB, da Silva LAB, Faccioli LH. Effects of 5-lipoxygenase gene disruption on inflammation, osteoclastogenesis and bone resorption in polymicrobial apical periodontitis. Arch Oral Biol. 2020 Apr;112:104670. doi: 10.1016/j.archoralbio.2020.104670. Epub 2020 Feb 6. PubMed PMID: 32058859. - Bastos LA, Silva FL, Thomé JPQ, Arnez MFM, Faccioli LH, Paula-Silva FWG. Effects of Papain-Based Gel Used For Caries Removal on Macrophages and Dental Pulp Cells. Braz Dent J. 2019;30(5):484-490. doi: 10.1590/0103-6440201902560. eCollection 2019. PubMed PMID: 31596333. - Ribeiro-Santos FR, Silva GGD, Petean IBF, Arnez MFM, Silva LABD, Faccioli LH, Paula-Silva FWG. Periapical bone response to bacterial lipopolysaccharide is shifted upon cyclooxygenase blockage. J Appl Oral Sci. 2019 Jun 3;27:e20180641. doi: 10.1590/1678-7757-2018-0641. PubMed PMID: 31166414; PubMed Central PMCID: PMC6546268. - Lorencetti-Silva F, Pereira PAT, Meirelles AFG, Faccioli LH, Paula-Silva FWG. Prostaglandin E2 Induces Expression of Mineralization Genes by Undifferentiated Dental Pulp Cells. Braz Dent J. 2019 Jun;30(3):201-207. doi: 10.1590/0103-6440201902542. Epub 2019 Jun 3. PubMed PMID: 31166389. - Daltoé MO, Paula-Silva FW, Faccioli LH, Gatón-Hernández PM, De Rossi A, Bezerra Silva LA. Expression of Mineralization Markers during Pulp Response to Biodentine and Mineral Trioxide Aggregate. J Endod. 2016 Apr;42(4):596-603. doi: 10.1016/j.joen.2015.12.018. Epub 2016 Feb 27. PubMed PMID: 26925520. - Paula-Silva FW, Petean IB, da Silva LA, Faccioli LH. Dual Role of 5-Lipoxygenase in Osteoclastogenesis in Bacterial-induced Apical Periodontitis. J Endod. 2016 Mar;42(3):447-54. doi: 10.1016/j.joen.2015.12.003. Epub 2016 Jan 7. PubMed PMID: 26778267. - Paula-Silva FW, Ghosh A, Arzate H, Kapila S, da Silva LA, Kapila YL. Calcium hydroxide promotes cementogenesis and induces cementoblastic differentiation of mesenchymal periodontal ligament cells in a CEMP1- and ERK-dependent manner. Calcif Tissue Int. 2010 Aug;87(2):144-57. doi: 10.1007/s00223-010-9368-x. Epub 2010 May 4. PubMed PMID: 20440482. - Paula-Silva FW, da Silva LA, Kapila YL. Matrix metalloproteinase expression in teeth with apical periodontitis is differentially modulated by the modality of root canal treatment. J Endod. 2010 Feb;36(2):231-7. doi: 10.1016/j.joen.2009.10.030. PubMed PMID: 20113780; PubMed Central PMCID: PMC2818380. - de Paula-Silva FW, Santamaria M Jr, Leonardo MR, Consolaro A, da Silva LA. Cone-beam computerized tomographic, radiographic, and histologic evaluation of periapical repair in dogs' post-endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Nov;108(5):796-805. doi: 10.1016/j.tripleo.2009.06.016. Epub 2009 Sep 5. PubMed PMID: 19734073. - de Paula-Silva FW, D'Silva NJ, da Silva LA, Kapila YL. High matrix metalloproteinase activity is a hallmark of periapical granulomas. J Endod. 2009 Sep;35(9):1234-42. doi: 10.1016/j.joen.2009.06.008. PubMed PMID: 19720222; PubMed Central PMCID: PMC2736957. - de Paula-Silva FW, Wu MK, Leonardo MR, da Silva LA, Wesselink PR. Accuracy of periapical radiography and cone-beam computed tomography scans in diagnosing apical periodontitis using histopathological findings as a gold standard. J Endod. 2009 Jul;35(7):1009-12. doi: 10.1016/j.joen.2009.04.006. PubMed PMID: 19567324. - Paula-Silva FW, Ghosh A, Silva LA, Kapila YL. TNF-alpha promotes an odontoblastic phenotype in dental pulp cells. J Dent Res. 2009 Apr;88(4):339-44. doi: 10.1177/0022034509334070. PubMed PMID: 19407154; PubMed Central PMCID: PMC3144054. |
||||
| Carga Horária: |
15 horas |
||||
| Tipo: | Obrigatória | ||||
| Vagas oferecidas: | 30 | ||||
| Ministrantes: |
Francisco Wanderley Garcia de Paula e Silva Maya Fernanda Manfrin Arnez |
 voltar |
|
Créditos © 1999 - 2025 - Superintendência de Tecnologia da Informação/USP |