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Atividade
| 126207 - Práticas no isolamento e análise de vesículas extracelulares |
| Período da turma: | 01/08/2024 a 31/07/2025
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| Descrição: | Programa resumido
Terminologia e definições de vesículas extracelulares; Métodos de isolamento de vesículas extracelulares; desafios ipostos pelos diferentes fluidos biológicos; Definições sobre coleta e armazenamento das amostras para o isolamento e análise de vesículas extracelulares; Estudos funcionais de vesículas extracelulares; análise dos conteúdos bioativos de vesículas extracelulares. Bibliografia: - Protocol for Exosome Isolation from Small Volume of Ovarian Follicular Fluid: Evaluation of Ultracentrifugation and Commercial Kits Shlomit Kenigsberg, Brandon A. Wyse, Clifford L. Librach, and Juliano C. da Silveira (Methods and Protocols, Methods in Molecular Biology, vol. 1660,DOI 10.1007/978-1-4939-7253-1_26) - Cell-secreted vesicles containing microRNAs as regulators of gamete maturation Juliano C da Silveira1, Ana Clara F C M de Ávila1, Hannah L Garrett2, Jason E Bruemmer2, Quinton A Winger2 and Gerrit J Bouma2 (http://joe.endocrinology-journals.org https://doi.org/10.1530/JOE-17-0200) - Isolation and Analysis of Exosomal MicroRNAs from Ovarian Follicular Fluid Juliano Da Silveira, Gabriella M. Andrade, Felipe Perecin, Flávio Vieira Meireles, Quinton A. Winger, and Gerrit J. Bouma (MicroRNA Protocols, Methods in Molecular Biology, vol. 1733, https://doi.org/10.1007/978-1-4939-7601-0_4) - Role of extracellular vesicles during oocyte maturation and early embryo development A. C. F. C. M. de A´ vilaA and J. C. da SilveiraA,B (Reproduction, Fertility and Development, 2020, 32, 56–64 https://doi.org/10.1071/RD19389) - Protocol to Study the Role of Extracellular Vesicles During Induced Stem Cell Differentiation Kelly C. S. Roballo, Carlos E. Ambrosio, and Juliano C. da Silveira (Next Generation Culture Platforms for Reliable In Vitro Models: Methods and Protocols, Methods in Molecular Biology, vol. 2273, https://doi.org/10.1007/978-1-0716-1246-0_3) - Isolation, Characterization, and MicroRNA Analysis of Extracellular Vesicles from Bovine Oviduct and Uterine Fluids Karina Can˜ o´ n-Beltra´ n, Meriem Hamdi, Rosane Mazzarella, Yulia N. Cajas, Claudia L. V. Leal, Alfonso Gutie´ rrez-Ada´ n, Encina M. Gonza´ lez, Juliano C. da Silveira, and Dimitrios Rizos (Next Generation Culture Platforms for Reliable In Vitro Models: Methods and Protocols, Methods in Molecular Biology, vol. 2273, https://doi.org/10.1007/978-1-0716-1246-0_16) - MicroRNAProfilingUsingaPCR-BasedMethod, Giuliana Ferronato, Marcela Cerezzetti, Alessandra Bridi, Cibele Prado, Gisaline dos Santos, Natália Bastos, Paola Rosa, Juliana Ferst, Juliano da Silveira (MicroRNAProfiling:MethodsandProtocols,MethodsinMolecularBiology,vol.2595,https://doi.org/10.1007/978-1-0716-2823-2_11) |
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| Carga Horária: |
160 horas |
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| Tipo: | Optativa | ||||
| Vagas oferecidas: | 8 | ||||
| Ministrantes: |
Juliano Coelho da Silveira |
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